RESUMO
The relationship between angiogenesis and fibrosis has been demonstrated in several pathological conditions, one of them being schistosomiasis. To observe whether suppression of angiogenesis would interfere with fibrosis, Thalidomide, an anti-angiogenesis drug, was administered during 30 consecutive days to mice with experimental schistosomiasis. Computerized morphometric measurements of fibrosis, and the counting of blood vessels from hepatic schistosomal lesions did not significantly differ when treated animals and their controls were compared at the end of the experiments. These rather unexpected results are presented under the understanding that they may be of interest during further studies on the anti-angiogenesis properties of thalidomide, and the relationship between angiogenesis and fibrosis.
Assuntos
Inibidores da Angiogênese/uso terapêutico , Cirrose Hepática/prevenção & controle , Fígado/irrigação sanguínea , Neovascularização Patológica/prevenção & controle , Esquistossomose mansoni/patologia , Talidomida/uso terapêutico , Animais , Modelos Animais de Doenças , Feminino , Fígado/parasitologia , Cirrose Hepática/parasitologia , Cirrose Hepática/patologia , Masculino , Camundongos , Neovascularização Patológica/parasitologiaRESUMO
The relationship between angiogenesis and fibrosis has been demonstrated in several pathological conditions, one of them being schistosomiasis. To observe whether suppression of angiogenesis would interfere with fibrosis, Thalidomide, an anti-angiogenesis drug, was administered during 30 consecutive days to mice with experimental schistosomiasis. Computerized morphometric measurements of fibrosis, and the counting of blood vessels from hepatic schistosomal lesions did not significantly differ when treated animals and their controls were compared at the end of the experiments. These rather unexpected results are presented under the understanding that they may be of interest during further studies on the anti-angiogenesis properties of thalidomide, and the relationship between angiogenesis and fibrosis.
Assuntos
Animais , Feminino , Masculino , Camundongos , Inibidores da Angiogênese/uso terapêutico , Cirrose Hepática/prevenção & controle , Fígado/irrigação sanguínea , Neovascularização Patológica/prevenção & controle , Esquistossomose mansoni/patologia , Talidomida/uso terapêutico , Modelos Animais de Doenças , Cirrose Hepática/parasitologia , Cirrose Hepática/patologia , Fígado/parasitologia , Neovascularização Patológica/parasitologiaRESUMO
Previous studies in mice with hypervitaminosis A have demonstrated that fat-storing cells (hepatic stellate cells-HSCs) participate in schistosomal granuloma fibrogenesis. The origin of such cells in portal areas, away from the Disse spaces, was herein investigated. HSCs were identified in frozen sections of the liver by means of Sudan III staining. They appeared as red-stained cells disposed along the sinusoids of normal mice, but were never found within portal spaces. However, in the chronically inflamed portal spaces of Capillaria hepatica-infected mice, Sudan III-positive cells were frequently present among leukocytes and fibroblast-like cells. Thus, there are no resident HSCs in portal spaces, but their presence there in chronic inflammatory processes indicates that they are able to migrate from peri-sinusoidal areas in order to reach the portal areas.
Assuntos
Espaço Extracelular , Hepatócitos/patologia , Cirrose Hepática/patologia , Sistema Porta/patologia , Animais , Feminino , Histocitoquímica , Camundongos , Ratos , Ratos WistarRESUMO
Previous studies in mice with hypervitaminosis A have demonstrated that fat-storing cells (hepatic stellate cells-HSCs) participate in schistosomal granuloma fibrogenesis. The origin of such cells in portal areas, away from the Disse spaces, was herein investigated. HSCs were identified in frozen sections of the liver by means of Sudan III staining. They appeared as red-stained cells disposed along the sinusoids of normal mice, but were never found within portal spaces. However, in the chronically inflamed portal spaces of Capillaria hepatica-infected mice, Sudan III-positive cells were frequently present among leukocytes and fibroblast-like cells. Thus, there are no resident HSCs in portal spaces, but their presence there in chronic inflammatory processes indicates that they are able to migrate from peri-sinusoidal areas in order to reach the portal areas.
